DNA sequencing of an E. coli colony isolated from a nozzle that had biofilm growth of both bacteria shows that conjugation has occurred. However, the transformed strain of E. coli did not express any new proteins. BLAST analysis shows that the gene for the L. monocytogenesprotein is incomplete in E. coli. Sequence comparison shows that E. coli has a truncated version of the gene that begins at nucleotide number 54 and continues to nucleotide 1257, meaning that no start codon is present.
Why would this interfere with transcription or translation?
1.DNA bases are species specific
2. no start codon for translation
3. E. coli DNA polymerase does not recognize an L. monocytogenes gene sequence
4. no promoter region for the transcription to begin at.
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DNA sequencing of an E. coli colony isolated from a nozzle that had biofilm growth of both bacteria...
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