To assist in completing this question, you may reference the Animated Technique Video - Isoelectric Focusing. What characteristics determine the position of a protein on an IPG strip at the end of isoelectric focusing? a. the protein's three‑dimensional structure b. local pH in the medium c. protein solubility d. the pI of the protein e. the molecular weight of the protein

The correct answers are options b and d.

Explanation:

Both the local pH of the medium and the isoelectric point or pI of the protein are the features, which helps in determining the protein position on the IPG strip at the termination of the isoelectric focusing.  

It is known that proteins refer to the biomolecules that get produced from amino acids. Amino acids are the components that comprise both carboxylic as well as amino group, and in isoelectric point the movement between the amino acid ceases.  

hydroxyl -oh

alcohols, always polar, can form hydrogen bonds with h2o molecules

carboxyl -cooh  

cargoxylic acids, has acidic properties which is a source of hydrogen ions, found in cells in ionized for with a charge of oh-1

carbonyl > co

ketone if within skeleton, aldehyde if group is at the end of skeleton, k & a may be structural isomers, it determines 2 sugars (ketose & aldose)

amino -nh2

amines, acts as a base, it can pick up h+ ions from surrounding solution, ionized with charge of 1+

methyl ch2

addition to rna/dna affects and gene expression

phosphate -opo3 ²⁻

organic phosphates, contributes to negative charge to, molecule that it is a part of, and potential to react with h20

sulfhydryl -sh

thiols, cross-linking stabilize the protein, and it is structure

we need additional information in order to determine the cause of death for anna garcia. there are at least 3 things with explanation included as to why we need them are as follows: