In the 1950s, Christian Anfinsen demonstrated the renaturation of the protein ribonuclease (RNase) in vitro. After reduction (to reduce the disulfide links) and the addition of urea (to denature the protein), the protein was in an unfolded state. After removing the urea and the reducing agent, the protein refolded, with greater than 90% activity. If the urea were removed after oxidation occurred, the protein had less than 5% activity. Why would the protein not refold correctly if the urea were removed after the reducing agent was removed? (In other words, what would happen if the urea were removed after oxidation?) Choose the best answer.

a. Urea would participate in weak bonding interactions with RNase, preventing oxidation of Cys.
b. The protein would not fully unfold (denature).
c. Disulfide bonds are not positioned correctly unless weak bonding interactions are present.
d. Contaminants in the RNase preparation would form covalent bonds with the protein, preventing reactivation.

You have been asked to lead a demonstration for the undergraduate microbiology lab course about the uses of negative staining when studying bacteria. a "negative" stain does not stain the bacterial cell itself but stains the space between cells. under magnification, the acidic (negativelycharged) nature of the stain will be repelled by the negatively charged bacterial cell wall and willleave the cell colorless in a stained background. negative stains are used primarily to reveal the presence of negatively charged bacterial capsules; therefore, they are also called capsule stains. encapsulated cells appear to have a halo surrounding them. the negative stain procedure does not require heat fixation, which limits any chances of alteration in bacterial cell shape and size. the bacterial suspension is added to a drop of stain, such as nigrosin or eosin, and drawn across the glass slide using a coverslip. nigrosin staining-not safranin staining-of klebsiella pneumoniae will allow for the visualization of the cell shape and the determination of the presence of a capsule. true/false

Ecologist have observed a sharp increase of sulfur dioxide in the air . what might be a possible consequence of excessive sulfur dioxide in the air ? a. acid rain b. ozone layer depletion c. ozone layer hole d. increase in sea levels

The nervous system interacts with the endocrine system by a. using hormones as connections b. sending nerve impulses directly to glands c. using neuroendocrine cells as connections d. sending neurotransmitters directly to neurons