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A research group discovers a new version of happyase, which they call happyase*, that catalyzes the chemical reaction HAPPY SAD. The researchers begin to characterize the enzyme In the first experiment, with [E_t] at 4 nM, they find that the V_max is 1.6 mu M s^-1. Based on this experiment, what is the k_cat for happyase *? (Include appropriate units.)
a. In another experiment, with [E] at l nM and [HAPPY] at 30 mu M, the researchers find that V_o = 300 nM s^-1. What is the measured K_m of happyase* for its substrate HAPPY? Include appropriate units.) Further research shows that the purified happyase* used in the first two experiments was actually contaminated with a reversible inhibitor called ANGER. When ANGER is carefully removed from the happyase* preparation and the two experiments repeated, the measured V_max in is increased to 4.8 mu M s^-1, and the measured K_m in is now 15 mu M, For the inhibitor ANGER, calculate the values of alpha and alpha' Based on the information given above, what type of inhibitor is ANGER?
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A research group discovers a new version of happyase, which they call happyase*, that catalyzes the...
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