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Biology, 10.03.2020 16:55 rafamoreura

A research group discovers a new version of happyase, which they call happyase, that catalyzes the chemical reaction: HAPPY ⇄ SAD (a) In the experiment with [Et] at 4 nM, they find that the V_max is 1.6 μ Ms^(-1). Based on this experiment, what is the k_cat for happyase*? (Include appropriate units.) (b) In another experiment, with [Et] at 1 nM and [HAPPY] at 30 μ M, the researchers find that V_0 = 300 nM s^1. What is the measured Km of happyase for its substrate HAPPY? Include appropriate units.) (c) Further research shows that the purified happyase used in the first two experiments was actually contaminated with a reversible inhibitor called ANGER. When ANGER is carefully removed from the happyase preparation, and the two experiments repeated, the measured Vmax in (a) is increased to 4.8 μ Ms^(-1), and the measured Km in (b) is now 15 μM. For the inhibitor ANGER, calculate the values of and. (d) Based on the information given above, what type of inhibitor is ANGER?

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