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Biology, 10.03.2020 16:31 Katyazamo

You are interested in examining the different kinetics of two isozymes of malate dehydrogenase (MDH). Isozymes are two similar enzymes that catalyze the same reaction but are from different genes or splice variants, giving them different properties. One of the MDH isozymes is located in the mitochondria, whereas the other is located in the cytoplasmic fraction. Your goal is to simply separate the two isozymes and test the samples in the partially purified state. Your research partner has centrifuged the cell extract at 10,000g for 10 minutes, but is not sure which fraction (pellet or supernatant) contains MDH. What should you tell your research partner?A. The pellet contains the heavy proteins, and MDH will be found in that fraction. B. Both the pellet (mitochondrial MDH) and the supernatent (cytoplasmic MDH) contain MDH. C. The pellet contains only the nuclear fraction, and thus both mitochondrial and cytosolic MDH proteins are still in the supernatant. D. The pellet has both the nuclear and the mitochondrial fractions, and thus the preparation is ruined.

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