The ATP that is generated in glycolysis is produced by substrate-level phosphorylation, a very different mechanism than the one used to produce ATP during oxidative phosphorylation. Phosphorylation reactions involve the addition of a phosphate group to another molecule. Sort the statements into the appropriate bin depending on whether or not they correctly describe some aspect of substrate-level phosphorylation in glycolysis.
A. One of the substrates is a molecule derived from the breakdown of glucose.
B. The phosphate group added to ADP to make ATP comes from free inorganic phosphate ions.
C. The enzymes involved in ATP synthesis must be attached to a membrane to produce ATP.
D. A bond must be broken between an organic molecule and phosphate before ATP can form.
E. An enzyme is required in order for the reaction to occur.