If the decolorizer is left on too long in the Gram-staining procedure, gram-positive organisms will be stained and gram-negative organisms will be stained .

If the decolorization is left on too long in the gram staining procedure,gram positive organisms will be stained Purple color and  gram negative organisms will be stained with no purple color.

Explanation:

Gram staining is used to identify bacterial species which are either gram positive or gram negative.

The cell wall of gram positive bacteria contain thick layer of peptidoglycans which hold the stain crystal violet and thus it becomes purple color where as the cell wall of gram negative bacteria  contain thinner layer of peptidoglycans which allows the washing of crystal violet stain and thus the cell wall is not stained with purple color.

this protocol provides a detailed procedure for agarose-based ip to purify and enrich the protein of interest.