An epidemiologist is investigating a new disease and observes what appear to be bacteria inside tissue cells in clinical samples from victims. The scientist wants to try to isolate the bacteria in the lab. What culture conditions are most likely to be successful?

Innoculation of cell tissues

Explanation: Inocculation is the growing of microbes in a nutrient based media. There are different ways of innoculating we have broths or Agar plates.

Media should be culture in an aseptic environment under a sterile condition to avoid contamination.

Bacteria, virus can be innoculated in a media.

The requirement for growth depends on the cell to be cultured. It includes,

amino acids

carbohydrates

vitamins

minerals

growth factors

hormones

CO2 or O2

pH buffer to maintain relatively neutral pH

osmotic pressure

temperature (typically 37 degrees C

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