Minor species. For an amino acid such as alanine, the major species in solution at pH 7 is the zwitterionic form. Assume a pKa value of 8 for the amino group and a pKa value of 3 for the carboxylic acid. Estimate the ratio of the concentration of the neutral amino acid spe- cies (with the carboxylic acid protonated and the amino group neutral) to that of the zwitterionic species at pH 7 (Section 1.3).