subject
Biology, 21.02.2020 01:33 jake7484

Forward primer: 5'-AGTCTACTCGTAACCGGTTACC-3' Reverse primer: 5'-TAAGGCATCATGGTAACCGGTT-3' The two primers above have the melting temperatures between 55C-80C. What is the reason that these two primers will not bind effectively to the region of DNA that we want to amplify?a. The length of the two primers is between 20-30 base pairs long. The length is too short and not very specific. b. The 3'-end of the reverse primer (3'-TTGGCCAATGG---5') is complementary to the forward primer (5'---AACCGGTTACC-3') and thus reduces the ability of the primers to bind to the targeted DNA sequence. c. The 3'-end of the forward primer is complementary to the 5'-end of the reverse primer and thus reduces the ability of the primers to bind to the targeted DNA sequence. d. The melting temperature range of the primers is similar to the DNA. e. Guanine and Cytosine make up 60% of the total base pairs.

ansver
Answers: 2

Other questions on the subject: Biology

image
Biology, 21.06.2019 20:00, ekautz9675
The process by which natural forces move weathered rock and soil brim one place to another is called
Answers: 1
image
Biology, 22.06.2019 02:00, billy1123
Which statement correctly describes a way that mutations increase the likelihood that a species will survive in a changing environment
Answers: 1
image
Biology, 22.06.2019 10:40, ari313
Which of the following factors would not contribute to allopatric speciation? a) a population becomes geographically isolated from the parent population. b) the separated population is small, and genetic drift occurs. c) the isolated population is exposed to different selection pressures than the ancestral population. d) different mutations begin to distinguish the gene pools of the separated populations. e) gene flow between the two populations is extensive.
Answers: 2
image
Biology, 22.06.2019 13:20, stefancvorovic1
Aragonite shells of clams, snails, or scleractinian corals may be preserved unaltered in cenozoic deposits, but they are generally dissolved or recrystallized in older deposits. true or false
Answers: 1
You know the right answer?
Forward primer: 5'-AGTCTACTCGTAACCGGTTACC-3' Reverse primer: 5'-TAAGGCATCATGGTAACCGGTT-3' The two pr...

Questions in other subjects:

Konu
Mathematics, 01.12.2020 06:30
Konu
Mathematics, 01.12.2020 06:30