You have a mixture of three proteins X, Y, and Z.

a. X is positively charged, has a mass of 100 kDa, it binds sugars.
b. Y is positively charged, has a mass of 200 kDa, it does not bind sugars.
c. Z is negatively charged, has a mass of 100 kDa, it does not bind sugars.

What will be the most efficient method to purify protein X from the mixture of X, Y, and Z?

i. washing
ii. drying
iii. ion-exchange chromatography
iv. gel filtration chromatography
v. affinity chromatography

iii. ion-exchange chromatography

Explanation:

We have to purify the proteins and column chromatography is used widely for the purification of proteins. Ion-Exchange chromatography is also a type of column chromatography.

When the sugar binding protein X passes through the column, the sugar and  proteins binds together by exchanging ions and coagulated out no matter what the nature of charge on proteins...

step 1 ammonium can be absorbed by plants, but some is converted into nitrates which are better for plants to absorb. this is called nitrification. 2. step 2 the deer eats the plants and uses the nitrogen-containing proteins for cell growth. 3. step 3 bacteria in the soil conduct nitrogen fixation to convert nitrogen gas from the atmosphere into ammonium. 4. step 4 some of the nitrates are absorbed back into plants, but denitrification breaks down the nitrates into nitrogen gas released back into the atmosphere. 5. step 5 nitrogen is found in the urine, which gets broken down into ammonium through the process of ammonification. 6. step 6 plants absorb the ammonium and begin assimilation, the process to add the nitrogen to protein.

answer: c.

explanation: