You wish to create a mutation in which you prevent access of rna polymerase to the gene. Which region of a gene would you target?

The promoter region/sequence

Explanation:

Promoter sequences/region is where the transcription factors and RNA polymerase must attach before the gene can be transcribed. SO if we mutate this sequence, the transcription factors and RNA polymerase won't recognize it thus would not attach and transcribe the targeted gene.

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