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(1.)Which question below do scientists need to answer, to determine whether CRISPR can be used to fight a new bacterium that is resistant to antibiotics? (Circle all that apply)
A. Where did the bacterium come from?
B. What sequence of nucleotides does the guide RNA need to include, to kill the bacterium?
C. What proteins do the virus’s guide RNA code for?

(2.) Which of the following system components is likely to be direct (immediate) cause the antibiotic-resistant bacterium’s own DNA to be destroyed?
A. the invasion of the bacteriophage (virus) into the bacterium
B. the action of the Cas-9 protein
C. the production of the Cas-9 protein
D. the coding of the guide RNA

1) What sequence of nucleotides does the guide RNA need to include, to kill the bacterium is the answer.

2). The action of the Cas-9 protein.

Explanation:

CRISPR is a genetic engineering technology used to detect and destroy DNA of the bacteriophage which have infected the bacterial cell previously.

The CRISPR are the sequences of bacteriophage DNA found in genome of the bacteria when bacteriophage has infected it before.

It alters the DNA sequences and modify the function of gene is bacteria.

Cas-9 is an endonuclease which cuts the DNA identified  by or complementary to CRISPR.

This system makes bacteria anti viral.

It is an example of acquired immunity.

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