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Biology, 19.02.2020 04:56 VamPL

A plasmid was cleaved with several restriction enzymes, individually and in combinations. The following fragment sizes (base pairs) were determined by agarose gel electrophoresis.
Eco RI 4363
Ava I 4363
Pvu II 4363
Pst I 4363
Eco RI-Ava I 2938 1425
Eco RI-Pst I 3609 754
Ava I-Pvu II 3722 641
Ava I-Pst I 2182 2181
Pvu II-Pst I 2820 1543
Eco RI-Pvu II 2297 2066
Make a restriction map based on this data. Indicate the distances between sites. Why is only one band detected in the Ava I-PstI co-digest

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