The introductory passage described a consortium consisting of ANME and a sulfur oxidizing bacteria. Which of the following would be important considerations in choosing the best technique to determine the number of living cells in a culture of the consortium?

Select the best answer.

A) Some fluorescent stains react differently based upon whether a cell has an intact cell membrane.
B) Phylogenetic stains can distinguish between species of Bacteria and species of Archaea.
C) DAPI can be used to stain DNA.
D) Cells present in low concentrations can be counted using microscopic cell counts, but they must be concentrated first.
E) Small cells are more difficult to see when doing microscopic cell counts.

C) DAPI can be used to stain DNA.

Explanation:

DAPI is a technique used for the effective quantification of bacteria by staining. This technique allows to visualize the DNA in living and fixed cells. It is a fluorescence technique, after this, quantify with the help of the electron microscope.

desert perennials often survive by remaining dormant during dry periods of the year, then springing to life when water becomes available. most annual desert plants germinate only after heavy seasonal rain, then complete their reproductive cycle very quickly.

this age may represent the age of the earth's accretion, of core formation, or of the material from which the earth formed. this dating is based on evidence from radiometric age-dating of meteorite material and is consistent with the radiometric ages of the oldest-known terrestrial and lunar samples.