2. Which two species are least closely related?
65
Number of Amino Acid Differences between Species
Species 1 Species 2 Species 3 Species 4
Species
1
0
74
Species 2
O
29
13
Species 3
29
0
81
Species 4 65
Species 2
74
13
81
A Species 1 & 2
B Species 1 & 3
C Species 2 & 3
D Species 3 & 4

Assume that you are interested in separating short (200-400 bp) dna molecules from a pool of longer molecules in the 10,000-20,000 nucleotide range. you have two recipes for making your polyacrylamide gels: one recipe uses 1.5 percent agarose and would be considered a “hard gel,” while the other uses 0.5 percent agarose and would be considered a loose gel. which gel should you use to separate the short (200-400 bp) dna molecules from a pool of longer molecules in the 10,000-20,000 nucleotide range?