Biology, 10.02.2020 19:47 discolldiane3708
In the 1940’s, Avery, MacCleod, and McCarty transformed nonencapsulated bacteria into encapsulated forms by growing the nonencapsulated cells in a cultre containing an extract made from dead encapsulated cells. The transformed cells produced colonies of encapsulated bacteria. Three different procedures and their results are outlined below.
Procedure I:
Extract made from dead encapsulated cells added to culture medium.
Nonencapsulated bacteria added to culture medium.
Results: Both nonencapsulated and encapsulated bacteria grow.
Procedure II:
Extract made from dead encapsulated cells treated with protein-degrading enzymes before adding extract to culture medium.
Nonencapsulated bacteria added to culture medium.
Results: Both nonencapsulated and encapsulated bacteria grow.
Procedure III:
Extract made from dead encapsulated cells treated with DNAse (an enzyme that selectively destroys DNA) before adding extract to culture medium.
Nonencapsulated bacteria added to culture medium.
Results: Only nonencapsulated bacteria grow.
What was the purpose of treating the extract with protein-degrading enzymes in Procedure II?
Answers:
A. To demonstrate that the transforming factor is an enzyme
B. To demonstrate that the transforming factor is not a protein
C. To destroy nucleic acids in the extract
D. To destroy any capsules in the extract
E. To prevent the extract from being contaminated by nonencapsulated bacteria
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