The early stage of protein purification often uses a procedure known as "salting out". this is typically done by treating the protein solution with ?

Answer is Ammonium sulfate.

Explanation:

The method of adding ammonium sulfate to a protein solution is known as precipitation. This is because the addition of ammonium sulfate will reduce or decrease the solubility of the protein. And as the ammonium sulfate is being increased, so also the solubility of the protein will be decreasing.

And at the long run, the protein will be precipitated. This is what is referred to as the 'salting out' procedure.

you did not attach the discourse, but i assume it to be the discourse on the origin and basis of inequality among men (the original titile in french is: "discours sur l'origine et les fondements de l'inégalité parmi les hommes"), also know as "second discourse". in this discourse, jean-jacques rousseau emphasizes that private property is the basis of all inequalities.

therefore, what enabled the old regime to maintain as long as it did was private property.

because the light is a great source of energy for the plants to survive. (i hope this )