Which of the following is not part of the normal process of cloning recombinant dna in a bacterial host cell?

a. restriction endonuclease digestion of plasmid dnaâs
b. production of new recombinant dna using dna ligase and digested plasmid dnas
c. joining of the target dna and the plasmid cloning vector in order to create a newly synthesized dna
d. transformation of bacterial host cells by the recombinant dna plasmids and selection using kanamycin
e. production of mrna from the cloned gene
f. none of the above

e. Production of mRNA from the cloned gene

Explanation:

During cloning in a bacterial host the recombinant DNA is replicated but not necessarily transcribed to mRNA. The promoters are controlled so that trancription and translation of the gene may occur under certain conditions.  For example addition of an inducer such as IPTG into bacterial media inducing protein expression via promoter binding.

answer: blah

explanation:

bc blah

answer: ice crystals in air

the water from the water body or another source of water gets evaporated as water vapor as an effect of heat on water. these water vapor rises in the upper layers of the earth atmosphere under high atmospheric pressure. the atmospheric pressure of these water molecules starts to decrease due to the effect of cold air. the invisible water vapor condenses into visible water droplets and ice crystals.these ice crystals and water droplets being less denser than the surrounding air floats on air as clouds.