The dilution streaking technique can take a bit of practice to master. which of the following represent sources of error that would likely result in failure to obtain isolated colonies? choose one or more:

a. forgetting to sterilize the loop prior to obtaining the inoculum
b. flaming the loop immediately after obtaining the bacterial sample
c. forgetting to flame the loop before streaking sequential sections of the plate
d, allowing the loop to cool for too long before proceeding d
e. not creating a small section of overlap across sequential sections

Answers are B, C, E.

Explanation:

Note that if the options picked as answers above are not followed , there will be error in obtaining the required or needed colonies.

For example , flaming the loop after getting the bacterial sample will cause the flame to kill the bacterial sample that will be about to streak on the plate, thereby creating space for contaminants to grow instead of the needed sample.

Also, if one forget to flame the loop, the contaminants present on the loop will grow together with the sample on the plate, and it will be very difficult to isolate the required organism.

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