Mutants 1 and 2 are two point mutations of riia. mutant 1 does not overlap with mutant 2. mutants 3 and 4 are two deletion mutations of riib. mutant 3 overlaps with mutant 4. mutant 5 is a deletion that overlaps mutants 2 and 4, but does not overlap mutants 1 and 3.

for each of the following crosses, you perform a high m. o.i infection of both mutants on e. coli strain b. then you harvest the lysate and perform a high m. o.i infection on e. coli strain k. finally, you dilute this second lysate and perform low m. o.i infections on separate plates with e. coli b and e. coli k.

predict the results by circling one answer per cross. select "equal number" if the e. coli b and e. coli k plates will have some plaques in equal proportions; select "no plaques" if both plates will show 0 plaques; select "can’t tell" if the information provided is insufficient to predict the result with certainty.

indicate if it it: more on e coli b, equal number, no plaques, or can't tell for each of the options below.

1x 2
1x 3
2x 4
3x 4
4x 5

the national environmental policy act (nepa) is a united states environmental law that promotes the enhancement of the environment and established the president's council on environmental quality (ceq). the law was enacted on january 1, 1970. to date, more than 100 nations around the world have enacted national environmental policies modeled after nepa.

desert perennials often survive by remaining dormant during dry periods of the year, then springing to life when water becomes available. most annual desert plants germinate only after heavy seasonal rain, then complete their reproductive cycle very quickly.

cells use diffusion to get substances in and out of themselves. that diffusion will occur across the cell membrane or the outer covering of the cell. because we are talking about the surface of the cell, surface area and volume are  important factors here. as a cell increases in size, its surface area and volume increase as well.  we call that the surface area to volume ratio (sa: vol).  but the catch is that  volume increases at a greater rate than surface  area. in other words, as a cell gets bigger, the sa: vol gets smaller. so a really small cell like bacteria is mostly cell membrane (surface) with very little contents (volume). a really big cell like an egg, is mostly insides with a super thin membrane. so what ends up happening is that the cell cannot efficiently diffuse all of the stuff it needs in/out to survive. therefore cell size is limited to about 100mm. so how do we make organisms bigger than 100mm? cells divide! by dividing and becoming multicellular, organisms are able to grow!   check out the diagrams i attached to see the effect of sa: vol. i hope this ! good luck!