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Biology, 17.12.2019 23:31 peacelillady1030

In order to identify genes that function together in genetic pathways researchers can perform modifier screens, looking for second site enhancers or suppressors of a mutant. which of the following is the most reasonable strategy for carrying out a suppressor screen in particular?

a. analyze the dna sequence near the mutation of interest, looking for sequences that may act as silencers
b. identify mutations that, when crossed with the original mutant, result in synthetic lethality
c. mutagenize the original mutant and look for new mutations that reduce the severity of the original mutant phenotype
d. screen for enhancer-trap strains that show gene expression differences in the original mutant
e. isolate mutants with phenotypes similar to the original mutant phenotype and then cross the mutants to identify the affected gene(s)

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