What are the two techniques used to create a dna profile in this experiment? what
function does each perform?

RFLP, AmpFLP are the two techniques which is used to create DNA profile

Explanation:

RFLP technique: RFLP technique stands for “Restriction Fragment Length Polymorphism”. It is molecular method of genetic analysis which allows to identified based unique pattern of restriction enzyme where DNA is cutting in specific regions. It requires large amount of sample. The costing is very high  

AmpFLP: The AmpFLP stands for amplified length polymorphism. It is PCR based tool. Firstly, it uses as restricted enzyme. It is cheaper than RFLP technique. It is used as genetic engineering

you are certainly talking about this picture:

a: is the label of the white square, it is showing the  replication fork, formed by helicase (d) ==> so it is the site of dna separation and replication 

b: is a new dna brand that is following directly the replication fork, so it is continuous ==> built continuously from 5' to 3

c: dna polymerase cannot replicate dna on its own, it needs the of their proteins, and one of them plays the role of a clamp which fixes  the dna with the dna template and increases  the processivity of the enzyme. ==>   stops polymerase from falling off the  template.

d: is the enzyme responsible for forming the replication fork (helicase) ==> unwinds and separates dna into two strands

e: is the delayed strand, it is built  in the opposite sense of the helicase, so dna will be built in fragments ==> built  fragments from 5' to 3 joins

and finally f: is the enzyme that builds  the new dna strand (dna polymerase) ==>   adds nucleotides to make a new dna helix.

credit: gradman555p98x9p

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