You add the following components (in the correct volumes and concentrations) to a tube to perform a pcr reaction buffer magnesium chloride forward and reverse primers dna polymerase (e. g.,taq) template dna pcr-grade water true or false: these components are sufficient to successfully carry out a pcr reaction. a. true b. false

b. False

Explanation:

These components are not sufficient to successfully perform a PCR reaction. Deoxynucleoside triphosphates (dATP, dGTP, dCTP, dTTP) are missing so that the PCR reaction can occur perfectly.

The PCR technique allows a specific fragment of the DNA molecule to be amplified thousands of times in just a few hours. This technique revolutionized research in molecular biology because it had taken a long time for DNA amplification. From PCR it is possible to obtain enough copies of a part of DNA to detect and analyze the sequence that is the target of the study.

For a PCR reaction to be performed a solution with some components must be prepared. These components are:

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