Bacteria lack rna-splicing machinery, which means they are unable to splice out introns from eukaryotic genes. to engineer a bacterium to produce a eukaryotic protein, it is necessary to synthesize a gene without introns. if you know the nucleotide sequence, you can

make a gene from the given mRNA without the introns.

Explanation:

Introns can be described as the non- coding regions of DNA. As they are non- coding, they are spliced out from the mRNA so that only the coding regions can be translated.

If we know a nucleotide sequence, then we can make a gene from it without added the introns. We can consider an mRNA strand and make a complementary strand from it, excluding the intron regions. Then from the DNA produced, we can make an mRNA without the introns and insert it into a bacterium.

explanation:

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