subject
Biology, 28.11.2019 20:31 kingemzy

If the egfp gene is approximately 700 bp in length, why do we predict the pcr product from positive clones in lab session 6c to be approximately 1200 bp in length? is it possible for a clone to have multiple copies of egfp inserted? could two copies be inserted? could three copies be inserted? if so, what would be the result of the pcr screen? if multiple copies of the egfp gene could be inserted, would it have more, less, or the same amount of fluorescence compared to a clone with a single copy of the egfp gene present? if your dna was not cut to completion during the restriction analysis of your transformants, what would you expect your gel to look like? would you still be able to distinguish positive from negative clones? when performing dna sequencing, you only use one primer per reaction. what would happen if you mistakenly added both a forward and reverse primer to a sequencing reaction? how would your data be affected?

ansver
Answers: 3

Other questions on the subject: Biology

image
Biology, 21.06.2019 21:30, natashalane8617
When explaining dominant and recessive traits to a younger family member, they respond, "well chances are i can probably taste ptc, since dominant traits are more common." how might you address this misconception?
Answers: 1
image
Biology, 21.06.2019 22:00, jimennacastillo15
What prevents odyssey from killing the sleeping cyclops
Answers: 1
image
Biology, 22.06.2019 03:00, Aminton737
What percentage of light hits earth's surface directly?
Answers: 3
image
Biology, 22.06.2019 09:00, maxicanofb0011
Which kind of worm has a closed circulatory system? a planarian b fluke c pinworm d an earthworm
Answers: 1
You know the right answer?
If the egfp gene is approximately 700 bp in length, why do we predict the pcr product from positive...

Questions in other subjects:

Konu
Mathematics, 14.11.2020 01:00
Konu
French, 14.11.2020 01:00
Konu
Mathematics, 14.11.2020 01:00
Konu
English, 14.11.2020 01:00