Afull-length eukaryotic gene is inserted into a bacterial chromosome. the gene contains a complete promoter sequence and a functional polyadenylation sequence, and it has wild-type nucleotides throughout the transcribed region. however, the gene fails to produce a functional protein.

a) list at least three possible reasons why this eukaryotic gene is not expressed in bacteria.

b) what changes would you recommend to permit expression of this eukaryotic gene in a bacterial cell.