The pcr (polymerase chain reaction) protocol that is currently used in laboratories was facilitated by the discovery of a bacterium called thermus aquaticus in a hot spring inside yellowstone national park, in wyoming. this organism contains a heat-stable form of dna polymerase known as taq polymerase, which continues to function even after it has been heated to 95oc. why would such a heat-stable polymerase be beneficial in pcr
a) each cycle includes a "hot" denaturation phase (95°c), which separates the hydrogen bonds that hold the strands of the template dna together.
b) each cycle includes a "hot" denaturation phase (95°c), which serves to sterilize the culture.
c)each cycle includes a "hot" denaturation phase (95°c), which activates the taq polymerase.
d)each cycle includes a "hot" saturation phase (95°c), which allows the primers to anneal to the target dna.
e)more than one of the above are correct.
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