Apoint mutation would be most likely to impact the protein produced, because

A point mutation would be most likely to impact the protein because during point mutation one or some bases in a nucleotide sequence of the open reading frame of mRNA get mutated.

Explanation:

The mutation in one nucleotide of an open reading frame(containing specifically arranged codons)of mRNA  is enough to change the normal open reading frame by changing only a single codon.

      Changing a single codon will alter the amino acid specific for that codon but alteration of a single amino acid can form an abnormal or disease causing protein.