Biology, 21.11.2019 20:31 lovecats12

To calculate cotransduction frequencies, you use bacteriophage to transduce dna from wild-type donor bacteria to recipient bacteria of various genotypes. for each experiment, you select for recipient cells that are prototrophic for alanine by plating cells on a master plate containing minimal medium + leucine, which allows all leu genotypes to grow. you then replica plate each master plate on minimal medium without leucine.

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To calculate cotransduction frequencies, you use bacteriophage to transduce dna from wild-type donor...