Biology, 21.11.2019 20:31 anime333

Describe the construction of a recombinant plasmid containing the gene for the green fluorescent protein (gfp) and the insertion of the plasmid into a bacterial cell by placing the steps in order
-ligate together the gfp gene and the plasmid to generate a recombinant plasmid-transform bacteria with the recombinant plasmid using electroporation
-use pcr to amplify the gene for gfp
-perform a restriction digestion of the gfp gene and the plasmid
-plate the bacterial cells, and screen for positive transformants

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Describe the construction of a recombinant plasmid containing the gene for the green fluorescent pro...