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Biology, 18.11.2019 23:31 natalie2sheffield

You are investigating a new bacterial operon that contains five regions (a, b, c, d and e) involved in coordinated regulation of transcription. one is the gene for a regulatory protein, one is the binding site for the regulatory protein, two produce structural enzymes (enzyme 1 and enzyme 2), and one is the promoter for the two enzyme genes. the table below shows data collected for five different bacterial strains. a "+" indicates that the structural enzyme is produced and a "-" indicates that the enzyme is not produced. the "signal" is a molecule that either represses or induces the operon. use the information to determine how the operon is regulated, and the identity of each region. strain enzyme 1 enzyme 1 enzyme 2 enzyme 2 -signal +signal -signal +signal a+b+c+d+e+ + - + - a-b+c+d+e+ - - - -a+b-c+d+e+ + + + +a+b+c-d+e+ + - - -a+b+c+d-e+ + + + +a+b+c+d+e- - - + -part emutations in region b and region d give the same results - when either of these regions is mutated, the operon is expressed under all conditions (called constitutive expression).you hypothesize that region b is the operator region and that region d is the repressor protein. to test your hypothesis, you create two partial diploid lines by introducing a f' plasmid with a wild-type lac operon: strain 1: f' a+ b+ c+ d+ e+ / a+ b- c+ d+ e+strain 2: f' a+ b+ c+ d+ e+ / a+ b+ c+ d- e+what experimental results would be predicted by your hypothesis? complete this table with the expected results by dragging the "+" and "-" signs to the appropriate locations.

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