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In one publication, the author implanted biomaterials subcutaneously in rats to test the biocompatibility of materials. at a few time points, the animals were sacrificed and the author performed h& e staining on the tissue samples. the thickness of fibrous capsule layer was then measured by calculating the average width between the two dash lines in a few h& e images of the same sample but different sections. what is the major problem of this measurement? (10 points)
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