Biology, 15.11.2019 21:31 20stirltrer

Genomic libraries are often screened using probes derived from organisms that are different than the one from which the library was generated. therefore, there are often mismatches between the probe and the most similar matching clones in the library. as a result, it's possible that no matching clones may initially be identified by hybridization. which of the follow, methods would likely increase the chances of identifying the most similar matching clones?
a) alter the hybridization conditions such that hybridizations with a few mismatches are tolerated.
b) create smaller probes using regions of your dna of interest that are thought not to be highly conserved.
c) create smaller probes using regions of your dna of interest that are thought to be more highly conserved.
d) use a cdna for your probe since all of the introns have been removed.

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Genomic libraries are often screened using probes derived from organisms that are different than the...