Arestriction map lists the locations of dna sequences that are cut by a particular restriction enzyme for a piece of dna, such as a chromosome or a plasmid. restriction maps are important when generating a construct for experimental use. digesting the dna sequence with the restriction enzymes will result in fragmented dna of predictable sizes, based on the restriction map, that allow a researcher to analyze if his or her construct was generated correctly when visualized using gel electrophoresis. use the linear restriction map to predict where bands would be expected on a gel if a digest is performed using the specified restriction enzymes. assume that there is enough restriction enzyme that every possible restriction site on each molecule of dna will be cut.
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