Biology, 05.11.2019 02:31 tonio638

Asample of cells with a total receptor concentration of 50 m was incubated with a ligand concentration of 100 m. following the incubation period, the concentration of unbound receptors was determined to be 15 m. what is the k d for the receptor-ligand interaction?

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Answer from: urieforlife

Answer : The value of K_d for the receptor-ligand interaction is $27.9\mu M$

Explanation :

The equilibrium reaction between receptor and ligand will be:

$R+L\overset{K_f}\rightarrow [RL]$ (forward reaction)

$[RL]\overset{K_d}\rightarrow R+L$ (backward reaction)

where,

R = receptor

L = ligand

[RL] = receptor-ligand complex

As we know that,

$K_f=\frac{1}{K_d}$

The expression of K_f is:

$K_f=\frac{[RL]}{[R][L]}$

As we are given that:

Total [R] = $50\mu M$

Total [L] = $100\mu M$

Free [R] = $15\mu M$

Bound [R] = $50-15=35\mu M$

Bound [L] = Bound [R] = $35\mu M$

Free [L] = $100-35=65\mu M$

Now put all the given values in above expression, we get:

$K_f=\frac{[RL]}{[R][L]}$

$K_f=\frac{(35)}{(15)(65)}$

$K_f=0.0358\mu M^{-1}$

Now we have to calculate the value of K_d for the receptor-ligand interaction.

$K_f=\frac{1}{K_d}$

$0.0358\mu M^{-1}=\frac{1}{K_d}$

$K_d=27.9\mu M$

Therefore, the value of K_d for the receptor-ligand interaction is $27.9\mu M$

Answer from: Quest

how do heroes resolve conflicts in beowulf?

question 15 options:

they sue each other in court

they get a mediator

the seek counseling

they get even or take revenge

explanation:

Answer from: Quest

an individual’s sex (i.e., whether they are a male or female) is determined by the sex chromosomes. most people have two sex chromosomes, one that is inherited from their mother and one that is inherited from their father. typically, females have two x chromosomes (xx) and males have one x chromosome and one y chromosome (xy). conditions caused by changes (“mutations”) in genes located on the x chromosome are considered x-linked.

x-linked recessive inheritance

most x-linked conditions are recessive. this means that in a person with two x chromosomes (most females), both copies of a gene (i.e., one on each x chromosome) must have a change or mutation whereas in a person with one x chromosome (most males), only one copy of a gene must have a mutation. a female with a mutation in one copy of a gene on the x chromosome is said to be a “carrier” for an x-linked condition. a male with a mutation in a gene on the x chromosome is typically affected with the condition. because females have two copies of the x chromosome and males have only one x chromosome, x-linked recessive diseases are more common among males than females. however, x-linked recessive diseases can occur in both males and females.

for x-linked recessive disorders, an unaffected carrier mother who has a mutation in a gene on the x chromosome can transmit either the x chromosome with this mutation or a “normal” x chromosome to her children. if the father is unaffected, none of her daughters will be affected and all of her daughters will be unaffected—since they will inherit at least one normal x chromosome from their father. however, each daughter will have a 50% chance of being an unaffected carrier like her mother and a 50% chance of both x chromosomes being normal.