subject
Biology, 05.11.2019 01:31 elopezhilario6339

In your research on amino acid biosynthesis in e. coli, you have identified three new auxotrophic mutants in a mutagenesis screen. each mutant can grow on minimal medium only if it is supplemented with a specific amino acid, as follows:
lys2 mutants require lysine.
gly3 mutants require glycine.
phe1 mutants require phenylalanine.
as part of your research, you decide to map the locations of the three mutant loci.

to make a rough map of the lys2, gly3, and phe1 loci, you perform an interrupted conjugation experiment between the following two strains:
hfr lys2+ gly3+ phe1+ amps × f- lys2- gly3- phe1- ampr
(note: amps indicates sensitivity to ampicillin. ampr indicates resistance to ampicillin.)
you interrupt conjugation after 1 min, 2 min, and 3 min and plate exconjugants on minimal medium containing ampicillin and various combinations of lysine, glycine, and phenylalanine. you then score the resulting plates for the presence of colonies.
based on the results shown below, what are the possible genotypes of the colonies that grew on each medium? answer the question by dragging one pink label to each pink target, one white label to each white target, and one blue label to each blue target. labels may be used once, more than once, or not at all.

ansver
Answers: 1

Other questions on the subject: Biology

image
Biology, 21.06.2019 15:30, synite
Order the steps of protein synthesis. amino acids are lined up in a sequence. rna is modified into mrna. dna is transcribed. a ribosome binds to mrna. chemical bonds are formed and a protein is produced.
Answers: 3
image
Biology, 21.06.2019 22:00, JeroMii
Which of the following is an advantage of solar energy?
Answers: 1
image
Biology, 22.06.2019 12:30, gonzaloc
Development has provided electrically for less money but what may be considered a cause of nuclear power
Answers: 3
image
Biology, 22.06.2019 17:50, jaylenmiller437
How does a catalyst influence a chemical reaction?
Answers: 1
You know the right answer?
In your research on amino acid biosynthesis in e. coli, you have identified three new auxotrophic mu...

Questions in other subjects: