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Biology, 01.11.2019 05:31 dianactorres

You are tasked with making a 40 nucleotide dna sequence via solid phase techniques from phosphoramidite building blocks. you know that your goal is to generate a minimum of 0.5 mmol of the final product. your individual coupling efficiencies are 96% which gives you a 20% overall yield. what is the minimum amount of the 3’ terminal building block you should start with to meet your goal?

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