Assume that a given plasmid vector to be used in a cloning experiment contains 4000 base pairs of dna. assume also that the restriction endonuclease cuj cuts this plasmid at the following sites (starting from an arbitrary zero point): 1000, 1500, and 3000. given complete digestion of the plasmid with the endonuclease so that only linear fragments are produced, what sizes of dna are expected?

answer: substrate concentration. enzymes work best when there is a high enough substrate concentration for the reaction they catalyse.

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answerpalpalis group;