Biology, 23.10.2019 04:50 Twitches

Researchers are trying to make a genetically modified (gm) pig that expresses bovine (cow) growth hormone to make it grow bigger. they isolated the bovine growth hormone gene, cut it and a plasmid with the same restriction enzyme, and mixed them together. they inserted the mixture into pig zygotes, but only normal pigs, not gm pigs, grew. what did they likely do wrong?

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In the given case, the researchers forgot to add DNA ligase after amalgamating the plasmid and gene together. DNA ligase refers to an enzyme, which repairs breaks or abnormalities in the backbone of double-stranded molecules of DNA.

It performs three prime activities, that is, it seals the recombination fragments, seals repairs in the DNA, and its associates or interlinks the Okazaki fragments, which are the small fragments of DNA produced at the time of the replication of the double-stranded DNA. As the mentioned procedure lacks the activities of DNA ligase, due to which only formation of normal pigs took place not the genetically modified pigs.

Answer from: Quest

answer: - few nutrients

high pressure

low temperatures

explanation: 1. few nutrients: open-ocean zone is located way far from the land, which is the main source of the essential nutrients.2. high pressure: pressure increases by 1 atmosphere for every 10 meters increase in depth.3. ample sunlight: a large fraction of the sunlight is reflected back to the atmosphere from the sea surface. 4. varying salinity: below the thermocline, the water is isolated from the atmosphere so the salinity remains stable over the year.5. low temperatures: the temperature of open-ocean zone ranges from a low of -2°c to an average of 17°c

Answer from: Quest

Atp is is our little "pocket" of energy. of course, if you have too little, then it means you have too little energy, and you'll pass out. the way to not have enough, is to have no energy sources (glucose, fats, proteins). you can't have too many atp molecules. cycles like the krebs cycle produce it, but if you have too much of any substrate (or too little for that matter), the cycle stops and atp is no longer made (it stops until substrate levels are such that the process can go on). in any case, this is unlikely since atp is constantly hydrolyzed and remade, even while you're asleep. you won't have too much. if you have zero, you are dead or dying.