Each of the four stages of cellular respiration occurs in a specific location in the cell. these locations permit precise regulation and partitioning of cellular resources to optimize the utilization of cellular energy.
match each stage of cellular respiration with the cellular location in which it occurs. locations may be used once, more than once, or not at all.
1. glycolysis
2. pyruvate oxidation
3. citric acid cycle
4. oxidative phosphorylation

The glycolysis occurs in the citosol, the pyruvate oxidation occurs in mitochondrial matrix. The citric acid cycle takes place in the mitochondrial matrix and the oxidative phosphorylation occurs in  the inner membrane of the mitochondria.

Explanation:

There are several enzymes located in the cytosol of the cell that degrade glucose to generate two pyruvate molecules (glucose that has 6 carbons is broken down into two pyruvate molecules, which have 3 carbons each)

The pyruvate generated from glycolysis leaves the cytosol and enters the outer membrane of the mitochondria and then passes to the mitochondrial matrix. Within the mitochondrial matrix, pyruvate undergoes oxidative decarboxylation where the enzyme pyruvate dehydrogenase is finally constituted.

Inside the mitochondrial matrix, the pyruvate dehydrogenase binds with coenzyme A, and this begins the citric acid cycle where they also bind to the oxal acetate and cycle the acid. The reactions release carbon as CO2 and NAD + and FAD + are reduced to NADH and FADH2, respectively.

In  the inner membrane of the mitochondria. NADH and FADH2 re-oxidize and release protons and electrons. Protons pass into the intermembrane space, while electrons enter the mitochondrial matrix after passing a complex protein system. As the proton output has a gradient in favor, there is a decrease in the pH in the mitochondrial matrix, but they are returned to its interior by an ionic channel. These protons join with O2 molecules and generate water. Next to this ionic channel is the ATP synthetase that uses energy to convert ADP + P into ATP

this protocol provides a detailed procedure for agarose-based ip to purify and enrich the protein of interest.

c

explanation: