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there is a specific for each enzyme and the location where the bind is called the two things that can cause an enzyme to denature is and

answer:

location where a substrate binds to an enzyme is called active site.

enzymes have an active site which is shaped so that only a molecule, known as the substrate,   with the correct shape (shape recognition) can link into the enzyme.   once the enzyme and the substrate become associated, the enzyme can increase the probability of a chemical reaction occurring by a number of mechanisms all explicable in terms of the principles of mass action and activation energy.  

when the substrate is bound at the active site, random movements of the substrate are prevented and the molecule becomes orientated in a fixed position on the enzyme thereby increasing the probability of reaction at specific sites on the substrate.   positioning the substrate on the active site may also distort the structure thereby producing a strain in one or more chemical bonds.

explanation:

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ans.

dna fingerprinting is defined as a process that determines the dna characteristics of an individual. these dna characteristics are unique among individuals as their fingerprints, so it is known as dna fingerprinting.

steps of dna fingerprinting includes extraction of dna from sample, amplification of dna, restriction digestion of amplified dna, and then analysis of dna by gel electrophoresis, respectively.

thus, the next step performed by the scientist is 'to cut the amplified dna (obtained by polymerase chain reaction) by restriction enzymes that cleave the dna into fragments and their analysis.'