Biology, 06.10.2019 01:30 josetheghost6313
Consider an experiment where your goal is to isolate bruton’s tyrosine kinase (btk) enzyme from a whole‑cell lysate. you have an affinity chromatography column with a tyrosine kinase inhibitor molecule covalently attached to the beads. the tyrosine kinase inhibitor binds and inhibits btk. as a result of the experiment, you are able to elute btk from the column, but in a mixture of other tyrosine kinases.
why are tyrosine kinases other than btk present in the eluate?
(a) the kinase inhibitor has low binding affinity.
(b) btk is rapidly degraded during cell lysis.
(c) the kinase inhibitor has low specificity.
(d) btk is inactive in the cell.
Answers: 2
Biology, 22.06.2019 11:30, kmchippps
According to theories of how life began, how did early organic molecules begin to separate from the outside world? a: specialized enzymes were required b: chains of amino acids created a barrier c: formation of microspheres or vesicles d: rna catalyzed the formation of membranes
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Biology, 22.06.2019 19:30, sarahelisabeth444
Explain what is meant by a river’s load, and describe how the load is carried.
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Consider an experiment where your goal is to isolate bruton’s tyrosine kinase (btk) enzyme from a wh...
Mathematics, 13.03.2020 03:36
Mathematics, 13.03.2020 03:36