List three ways by which eukaryotes process mrna after transcription.

The post transcription modification include three ways i.e., capping, polyadenylation and splicing.

1. 5' Capping- mRNA capping is catalyzed by capping enzyme which is associated with RNA polymerase II. This enzyme adds the guanosin residue to 5' end of mRNA then methyl transferase adds methyl group to N⁷ position this guanine. Capping protects mRNA from degradation by enzymes presents in the cell.

2. Polyadenylation: In polyadenylation many adenosine residue is added at 3'end of mRNA by poly(A) polymerase. This poly A tail helps in exporting mRNA out of nucleus and binding of translational factors.

3. Splicing: In splicing process the non coding introns are removed from RNA so that only coding region i.e., exons are left in mRNA. It is important to remove unwanted sequence like introns which hinders in protein formation.

step 1 ammonium can be absorbed by plants, but some is converted into nitrates which are better for plants to absorb. this is called nitrification. 2. step 2 the deer eats the plants and uses the nitrogen-containing proteins for cell growth. 3. step 3 bacteria in the soil conduct nitrogen fixation to convert nitrogen gas from the atmosphere into ammonium. 4. step 4 some of the nitrates are absorbed back into plants, but denitrification breaks down the nitrates into nitrogen gas released back into the atmosphere. 5. step 5 nitrogen is found in the urine, which gets broken down into ammonium through the process of ammonification. 6. step 6 plants absorb the ammonium and begin assimilation, the process to add the nitrogen to protein.