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Biology, 13.09.2019 00:30 jeanbeansmith2p2ofty

You are studying mutations in a bacterial gene that codes for an enzyme whose amino acid sequence is known. in the wild-type protein, proline is the fifth amino acid from the amino terminal end. in one of your mutants with nonfunctional enzyme, you find a serine at position number 5. you subject this mutant to further mutagenesis and recover three different strains. strain a has a proline at position number 5 and acts just like wild type. strain b has tryptophan at position number 5 and also acts like wild type. strain c has no detectable enzyme function at any temperature, and you can't recover any protein that resembles the enzyme. you mutagenize strain c and recover a strain (c-1) that has enzyme function. the second mutation in c-1 responsible for the recovery of enzyme function does not map at the enzyme locus. a. what is the nucleotide sequence in both strands of the wild-type gene at this location? b. why does strain b have a wild-type phenotype? why does the original mutant with serine at position 5 lack function? c. what is the nature of the mutation in strain c? d. what is the second mutation that arose in c-1?

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