While creating a piece of artwork, lupita becomes curious about the ink she using to create her art. she wonders if the ink is pure substance or a mixture. the ink separates when a chromatography experiment is performed on it , leaving multiple marks of different colors along the chromatography paper. when the ink is left out for twenty four hours, the ink settles into individual components. using her observations of the properties of the ink, the student can classify it as

mixture

Explanation:

Chromatography is a process to separate the various substances present in mixtures based on their properties such as charges, size, affinity, etc.  

A pure substance cannot be separated into various substances as it consists of a single type of substance only. A mixture consists of two or more substances that are separated by various physical and chemical methods.  

The ink was separated into different colors on the chromatographic paper suggesting that it was a mixture of various colors. Therefore, the ink was a mixture, not a pure substance.