Which of the following is not required for the proper use of molecular beacons?
a. complementarity between the template dna and the probe sequence
b. detachment of a separate hybridized probe molecule from the template dna
c. illumination of the hybridized beacon to detect fluorescence
d. proximity-based quenching of the fluorophore prior to beacon hybridization

b. Detachment of a separate hybridized probe molecule from the template DNA

Explanation:

Molecular beacons are a type of genetic probe that enables the hybridization of oligonucleotides. These molecules have a flourishing component that binds to a nucleotide sequence and allows the identification of this nucleotide sequence in DNA or RNA without the release of radioactivity. For the use of these molecular beacons to be possible, one needs complementarity between the model DNA and the probe sequence,  illumination of the hybridized beacon to detect fluorescence and proximity-based quenching of the fluorophore prior to beacon hybridization.

because of