When using an eia to study microtubules or other structures inside a cell, we first chemically fix the cell and then treat the cells with alcohol. what is the purpose of this alcohol treatment?
it makes holes in the cell membrane large enough for antibodies to pass.
it makes the membrane sticky so antibodies will bind and be taken up by receptor-mediated endocytosis.
it removes negative charges from the membrane, which would otherwise repulse the antibodies.
it prevents nonspecific binding of the antibodies to the cell membrane.