Suppose that a thin-layer chromatography plate the solvent front migrates a distance of 14 cm, and two steroid spots move distances of (a) 6 cm and (b) 12 cm. calculate the rf values for each steroid.

A)

B)

Explanation:

RF value in thin-layer chromatography plate is defined as the ratio of distance moved by the steroid spot (A) to the distance migrated by solvent front(B)

Hence, mathematically

------Equation (A)

a) Here cm and cm

Substituting the given values in above equation, we get -

b) Here cm and cm

Substituting the given values in above equation, we get -

that scientist would be robert hooke

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